Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

Summary Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous factors. In this study, we analyze physiologic prokaryotic ribosome isolating bona fide pre-50S subunits from an Escherichia coli strain with factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals network interacting factors consisting YjgA, RluD, RsfS, ObgE on immature subunit. addition, our study provides mechanistic insight into how GTPase concert other factors, facilitates maturation 50S functional core both conserved divergent evolutionary features between prokaryotes eukaryotes.